Composite

Part:BBa_K5453005:Design

Designed by: Peipei Liu   Group: iGEM24_SZSAIS-Shenzhen   (2024-09-18)


pBAD-Gatz-PGP-Trrnb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 658
    Illegal NgoMIV site found at 784
    Illegal AgeI site found at 329
    Illegal AgeI site found at 1556
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of the two genes under the pBAD promoter is Gatz, PGP.


Source

References